Fig. 5: The protein level of MAT2A is decreased in liver tumors.

The heatmaps showing the transcriptome (A) and proteome (B) in different mouse tumor models. WT, wild type mice. ICR and C57 were two breeds of mice. C Western blotting showing the protein levels between non-cancer tissue and cancer tissue in Myc-Ctnnb1 driving tumor model. The tissues were ground with steel balls and boiled with SDS loading buffer. D The relative amounts of NADP⁺ in non-cancer tissue and cancer tissue in Myc-Ctnnb1 driving tumor model. The amounts of NADP⁺ were detected by Coenzyme II NADP(H) Content Assay Kit (Sangon Biotech, Cat. #D799249). The contents of NDAP⁺ in non-cancer tissue were normalized as 1. The data were represented by the mean ± SEM (N = 3 independent replicates). The p value was determined by two-sided paired t-test. E The relative amounts of NADP⁺ in MAT2B rescued and MAT2BΔ3541 rescued U2OS cells treated with DMSO, KG (alpha-Ketoglutaric, 5 mM for 48 h) and DMKG (dimethyl-α-ketoglutarate, 5 mM for 48 h). The amounts of NADP⁺ were detected by Coenzyme II NADP(H) Content Assay Kit (Sangon Biotech, Cat. #D799249). The contents of NDAP⁺ in WT MAT2B rescued U2OS treated with DMSO were normalized as 1. The data were represented by the mean ± SEM (N = 3 independent replicates). The p value was determined by two-sided paired t-test. F Western blotting results showing MAT2A decreased in WT MAT2B rescued but not MAT2BΔ35-41 rescued U2OS cells after being treated with KG and DMKG. Cell extracts were analyzed by Western blotting using the indicated antibodies. The assay was repeated three times with similar results.