Fig. 5: DH-induced mitochondrial DNA (mtDNA)-mediated pyroptosis. | Cell Death & Disease

Fig. 5: DH-induced mitochondrial DNA (mtDNA)-mediated pyroptosis.

From: Dronedarone hydrochloride (DH) induces pancreatic cancer cell death by triggering mtDNA-mediated pyroptosis

Fig. 5

A Morphology observation of PANC-1 after treatment of vehicle, DH or DH combined ddC. Scale bar, 100 μm. B Released LDH activity in PANC-1 cells after treatment of DH or DH combined with ddC. n = 6. C Representative images of Western Blot images of pyroptosis-related proteins in PANC-1 cells after different treatment. D–F Quantification of cleaved-GSDMD, HMGB1, and cleaved-IL1β protein expressions in Fig. 5C. n = 3. G Representative images of MitoSox staining results in PANC-1 cells after different treatments. Scale bar, 100 μm. Red, MitoSOX; Blue, DAPI. H Quantification of MitoSOX positive staining area in images in figure (G). n = 5. Data was shown as mean ± SD. Mutiple t-test are followed by Bonferoni correction was used. *p < 0.05; **p < 0.01; ***p < 0.001.

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