Fig. 5: MAT1A enhances the glycolysis of NSCLC cells through CCND1.
From: MAT1A activation of glycolysis to promote NSCLC progression depends on stabilizing CCND1
The metabolic profile of glycolysis in MAT1A knockdown A549 cells was analyzed, including (A) glucose utilization, lactic acid production, PA, PGK, GAPDH activity and (B) expression of pivotal glycolytic enzymes (PKM2, ALDOC, ADH6). C, D Measurement of glucose utilization, lactic acid production and (D) expression of pivotal glycolytic enzymes (PKM2, ALDOC, ADH6) in A549 cells (NC, as negative control; MAT1A-overexpressing; CCND1 knockdown; MAT1A-overexpressing and CCND1 knockdown). E, F MAT1A-overexpresed A549 cells treated with AKT inhibitor (perifosine) and measurement of (E) glucose utilization, lactic acid production and (F) expression of pivotal glycolytic enzymes (PKM2, ALDOC, ADH6). G, H MAT1A-overexpresed A549 cells treated with glycolysis inhibitor 2-deoxy-d-glucose (2-DG) and measurement of (G) glucose utilization, lactic acid production and (H) expression of pivotal glycolytic enzymes (PKM2, ALDOC, ADH6). Results were shown as mean ± SD. *p < 0.05, **p < 0.05, ***p < 0.001.
