Fig. 4: Knockdown of GRHL2 effectively induced the transition of epithelial cells into a mesenchymal state.
From: GRHL2 regulates keratinocyte EMT-MET dynamics and scar formation during cutaneous wound healing
A Wound healing assay to determine cell migration. Photos of the scratch for analysis were taken at 0 h, 8 h, 12 h, 24 h and 32 h in monolayers of control and GRHL2 KD keratinocytes, respectively. The remaining scratch area was determined based on microscopic images using ImageJ. The data are presented as means ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n = 6. B Expression levels of EMT-related markers in normal and GRHL2 KD keratinocytes according to western blotting for fibronectin, E-cadherin, N-cadherin, ZO-3 and GRHL2. C Quantification of protein bands from the western blot in B. D Expression levels of epithelial and mesenchymal genes according to RT-qPCR in control and GRHL2 KD keratinocytes. The data are presented as means ± SD, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. E Immunofluorescence analysis of vimentin, fibronectin, E-cadherin and ZO-1 in control and GRHL2 KD keratinocytes. Blue indicates the cell nuclei. Scale bar = 40 μm (20×) or 20μm (40×). TJP3 (ZO-3), GJA1 (gap junction protein alpha 1), FN1 (fibronectin), CDH2 (N-cadherin), TNC (tenascin-C).
