Fig. 5: GRHL2 overexpression promoted the MET of Keratinocytes.
From: GRHL2 regulates keratinocyte EMT-MET dynamics and scar formation during cutaneous wound healing
A Schematic of doxycycline (Dox)-inducible overexpression of GRHL2 in EMT keratinocytes. B Morphological changes of EMT keratinocytes following GRHL2 overexpression. C Overexpression efficiency of GRHL2 in EMT keratinocytes according to RT-qPCR. D Expression levels of EMT-related markers according to RT-qPCR in EMT keratinocytes treated with Dox or left untreated. Error bars represent standard deviations, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. JUP (junction plakoglobin), IVL (involucrin), FN1 (fibronectin), VIM (vimentin), CDH2 (N-cadherin), TNC (tenascin-C). E Expression levels of fibronectin and E-cadherin according to western blot analysis in EMT keratinocytes treated with Dox or left untreated. F Quantification of protein bands from the western blot in E.
