Fig. 3: DOCK8 point mutations decrease B cell spreading, BCR cluster formation and BCR signalosome recruitment.
From: DOCK8 gene mutation alters cell subsets, BCR signaling, and cell metabolism in B cells
Purified splenic B cells from WT and DOCK8 Mut mice were activated with AF647-Fab-goat-anti-mouse Ig (M + G) (10 μg/ml) for 3 min, 5 min, and 7 min, then fixed, permeabilized, and stained for markers following. A, C Representative total internal reflection fluorescence microscopy (TIRFm) images of pSYK and pSHIP1 (100× objective, scale bar = 5 μm). B, D MFI of pSYK and pSHIP-1 was performed using NIS-Elements AR 5.0.1 software. Data shown in the figures are representative of at least three independent experiments. All data are presented as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001; two-tailed unpaired t-test).
