Fig. 4: Loss of AEP led to elevated lysosomal pH by modulating V-ATPase stoichiometry/assembly. | Cell Death & Disease

Fig. 4: Loss of AEP led to elevated lysosomal pH by modulating V-ATPase stoichiometry/assembly.

From: Asparagine endopeptidase regulates lysosome homeostasis via modulating endomembrane phosphoinositide composition

Fig. 4

A Lysosensor labeled lysosomes in WT cells but not in AEPKD cells. WT and AEPKD MDA-MB-231 cells were labeled with 2.5 µM lysosensor for 10 min and imaged live. B Ratiometric pH measurement showing elevated lysosome pH in AEPKD cells. Data are mean ± s.d.; n = 5. C Partition of V-ATPase subunits in membrane extract (ME) and cytoplasm extract (CE) in WT and AEPKD cells in the presence or not of 100 nM BA1. ME and CE were analyzed by immunoblotting with indicated antibodies. V-ATPase V0D1, Rab5, and LC3 were used as membrane control. D Quantification of protein ME/CE ration in (C). Results shown are from representative experiments. **p < 0.01; ***p < 0.001 by two tailed t-test.

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