Fig. 1: TMEM232 is an evolutionarily conserved testis-enriched protein.
From: TMEM232 is required for the formation of sperm flagellum and male fertility in mice
A RT-qPCR and B western blotting analysis of TMEM232 expression in multiple tissues from mice. The representative image of biological duplicates is shown. C RT-qPCR and D western blot analysis of TMEM232 in mouse testes on various postnatal days. The image represents two independent experiments. E RT-qPCR analysis of Tmem232 in different fractions of spermatogenic cells. PS (pachytene spermatocytes), RS (round spermatids) and ES (elongating spermatids) were from mice at 2-months-old (n = 6), SG (spermatogonia) and SE (somatic Sertoli cells) were isolated from mice at postnatal day 6 using the STA-PUT method (n = 18). F The protein amount of TMEM232 in different fractions of spermatogenic cells. The image is a representation of two independent experiments with similar results. GADPH and α-Tubulin served as loading controls for RT-qPCR and western blotting, respectively. n = 3 for each sample. G Immunofluorescent staining of TMEM232 (green), phalloidin (F-actin, red), and DAPI (Nucleus, blue). GC-1, MGC-803, and HeLa cells were transfected with a pEGFP-N2-TMEM232 plasmid. Scale bar: 10 µm.
