Fig. 3: Tmem232 deficiency disrupts the midpiece-principal piece junction in mice sperm.
From: TMEM232 is required for the formation of sperm flagellum and male fertility in mice
A Morphology of mouse sperm obtained from the caudal epididymes. Tmem232−/− sperm flagella showed a gap between the midpiece and principal piece. Magnification: 20×, scale bars: 5 µm. No change was observed in the expression of PNA (B) and DM1A (C) between Tmem232−/− and Tmem232+/+ mouse sperm. (red, detected protein, blue, DAPI; scale bar: 1 µm). D Transmission electron microscopy (TEM) analysis revealed that the annulus of Tmem232−/− mouse sperm lost contact with the MS; However, this was not the case for Tmem232+/+ mouse sperm. An annulus, FS fibrous sheath, Mi mitochondrion; Scale bar: 1 µm or 500 nm.
