Fig. 1: TAp63α is accumulated in dimeric forms following CDDP injection.
From: Oocyte death is triggered by the stabilization of TAp63α dimers in response to cisplatin
A Immunoblot analysis of TAp63α on BN-PAGE using ovarian extracts from mice exposed to 0.45 Gy irradiation. Ovaries were harvested at 0, 2, 4, and 6 h post-exposure. The molecular weights of the TAp63α dimer (480 kDa, a black arrowhead) and tetramer (720 kDa, a red arrowhead) are indicated on the BN-PAGE. B Histological analysis with H&E staining on ovarian samples from mice exposed to 0.45 Gy irradiation. Intact primordial follicles are marked with blue arrowheads, while apoptotic follicles are marked with green arrowheads. C Immunoblot analysis of TAp63α on BN-PAGE, and TAp63α and GAPDH on SDS-PAGE, using ovarian extracts from mice treated with either solvent (Control, Ctr) or 5 mg/kg CDDP at 8, 17, and 21 h post-injection. Phosphorylated and unphosphorylated bands on SDS-PAGE are marked with red and black arrowheads, respectively. D Histological analysis with H&E staining, immunofluorescence assay for TAp63α, and TUNEL assay in ovarian samples from mice treated with either solvent (Control, Ctr) or CDDP at 8, 17, and 21 h post-injection. Intact primordial follicles are marked with blue arrowheads, and apoptotic ones with green arrowheads. Insets show TAp63α expression at each time point, with TUNEL-positive oocytes indicated by white arrowheads. E Quantification of TAp63α Intensity. F Survival rate of primordial follicles. G Quantification and calculation of TUNEL-positive primordial follicles in each ovarian samples. Statistical significance: n.s. not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
