Fig. 5: Ubiquitin-mediated degradation of TAp63α occurs in oocytes within primordial follicles.
From: Oocyte death is triggered by the stabilization of TAp63α dimers in response to cisplatin
A Immunoblot analysis of TAp63α and GAPDH in ovaries cultured with solvent (Control, Ctr) or PYR-41 for 6 h. B Relative intensity of TAp63α. Each dot represents a biologically distinct female ovary (n = 3 per group). C Immunofluorescence assay of TAp63α in ovaries. D Intensity analysis of TAp63α in oocytes (n = 20) of primordial follicles from ovaries post-culture for 6 h (n = 3). E Histology of ovaries 48 h after exposure to PYR-41. F Total number of primordial follicles per ovaries following a 48-h culture period. Statistical significance: n.s. not significant; **p < 0.01.
