Fig. 4: TNFAIP2 promotes HIF1α transcription and angiogenesis in vitro via ERK signaling. | Cell Death & Disease

Fig. 4: TNFAIP2 promotes HIF1α transcription and angiogenesis in vitro via ERK signaling.

From: TNFAIP2 promotes HIF1α transcription and breast cancer angiogenesis by activating the Rac1-ERK-AP1 signaling axis

Fig. 4

A ERK1/2 inhibition abolished TNFAIP2-induced HIF1α upregulation. HCC1806 cells with stable TNFAIP2 overexpression were treated U0126 (10 μM, 12 h) and exposed to 1% O2 for 4 h. Cell lysates were harvested for WB analysis. B ERK1/2 inhibition abolished TNFAIP2-induced HIF1α and VEGFA mRNA upregulation. HCC1806 cells with stable TNFAIP2 overexpression were treated U0126 (10 μM, 12 h). Cell lysates were harvested for real-time PCR analysis. C ERK2 knockdown abolished TNFAIP2-induced HIF1α upregulation in response to hypoxia. D ERK2 knockdown abolished TNFAIP2-induced HIF1α and VEGFA mRNA upregulation. E, F ERK1/2 inhibition impeded the TNFAIP2 overexpression-induced migration of HUVECs by the wound-healing assay. Representative images are shown. G, H ERK1/2 inhibition impeded the TNFAIP2 overexpression-induced tube formation of HUVECs. Representative images are shown. I, J ERK2 knockdown impeded the TNFAIP2 overexpression-induced migration of HUVECs by the wound-healing assay. Representative images are shown. K, L ERK2 knockdown impeded the TNFAIP2 overexpression-induced tube formation of HUVECs. Representative images are shown. Scale bar, 200 μm, in the histogram, the bars represent the mean ± SD (n = 3), *P < 0.05, **P < 0.01 and ***P < 0.001; ns not significant.

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