Fig. 2: Overexpression of MDM4 inhibits ferroptosis in p53 mutant colon cancer cells.
From: MDM4 inhibits ferroptosis in p53 mutant colon cancer via regulating TRIM21/GPX4 expression
A Colony formation assay showed that HT29 cells overexpressing MDM4 resisted to ferroptosis induced by RSL3 (n = 3). B Microscopic images demonstrating cell morphology changes in HT29 cells after RSL3 treatment with or without MDM4 overexpression (n = 3). C Cell viability of HT29 cells treated with different doses of RSL3 alone, or in combination with Fer-1 or Z-VAD for 48 h (n = 3). D Effect of MDM4 overexpression on GSH/GSSG ratio in HT29 cells (n = 3). E Flow cytometry analysis of ROS production in MDM4 overexpression cells treated by RSL3 alone or with Fer-1. F MDA content measured in MDM4 overexpression cells treated by RSL3 alone or with Fer-1 (n = 3). G Ultra-high resolution microscopy images showing mitochondrial morphology in SW480 cells overexpressing MDM4 after RSL3 treatment. ns: P > 0.05; *P < 0.05; **P < 0.01. A, C, F One-way ANOVA and D T test were used for statistical analysis.
