Fig. 6: MDM4 upregulates GPX4 through TRIM21.
From: MDM4 inhibits ferroptosis in p53 mutant colon cancer via regulating TRIM21/GPX4 expression
A Immunoprecipitation of Myc-tagged MDM4 detected interaction with GPX4. B Immunoprecipitation of Flag-tagged GPX4 detected interaction with MDM4. C Effect of MDM4 overexpression on the expression level of the GPX4-interacting E3 ubiquitin ligase identified by mass spectrometry assay in HT29 cells. Immunoprecipitation of D Flag-tagged GPX4 detects interaction with Myc-TRIM21, E Myc-tagged TRIM21 detects interaction with Flag-GPX4, F Flag-tagged GPX4 detects interaction with endogenous TRIM21, G Myc-tagged TRIM21 detects interaction with endogenous GPX4, H Myc-tagged TRIM21 detects interaction with HA-MDM4, I HA-tagged MDM4 detects interaction with Myc-TRIM21, J HA-tagged MDM4 detects interaction with endogenous TRIM21.
