Fig. 7: MDM4 upregulates GPX4 protein expression levels through TRIM21.
From: MDM4 inhibits ferroptosis in p53 mutant colon cancer via regulating TRIM21/GPX4 expression
A Expression levels of TRIM21 in colon cancer compared to normal tissues from TCGA and GTEx databases. B Western blot showed the effect of TRIM21 overexpression on GPX4 protein expression levels in HT29 cells. C Western blot showed the effect of TRIM21 knockdown on GPX4 protein expression levels in HT29 cells. D Ubiquitylation assay showed that TRIM21 promoted the polyubiquitination transition of GPX4 from K48 to K63. E Ubiquitylation assay showed the effect of Flag-GPX4 mutants on the GPX4 ubiquitination change induced by TRIM21. F Western blot showed the change in GPX4 expression after TRIM21 knockdown in HT29 cells overexpressing MDM4. G Flow cytometry analysis of ROS production in TRIM21 overexpressing cells. H MDA content measured in TRIM21 overexpressing cells (n = 3). I Flow cytometry analysis of ROS production in TRIM21 knockdown cells. J MDA content measured in TRIM21 knockdown cells (n = 3). ns: P > 0.05；*P < 0.05; **P < 0.01; ***P < 0.001. H, J One-way ANOVA were used for statistical analysis.
