Fig. 8: Mouse experiments demonstrate that inhibition of MDM4 enhances the sensitivity of colon cancer graft tumors to RSL3.
From: MDM4 inhibits ferroptosis in p53 mutant colon cancer via regulating TRIM21/GPX4 expression
A Western blot showed the effect of NSC146109 on MDM4 protein expression and RSL3 on GPX4 protein expression. B Schematic diagram of the intraperitoneal drug administration process in nude mice. C Diagram illustrating tumor formation in nude mice injected with HT29 cells after drug treatment. D Changes in body weight of nude mice in each treatment group. E Subcutaneous tumor sizes at different time points after inoculation in nude mice. F Graph showed subcutaneous tumors collected from nude mice after necropsy. G Weights of subcutaneous tumors collected from nude mice after necropsy. H Immunohistochemical analysis of protein expression in subcutaneous tumors from nude mice. I Flowchart of tissue-specific expressing MDM4 mouse construction. J Expression levels of TRIM21 and GPX4 in colon tissues from tissue-specific expressing MDM4 mice. ns: P ≥ 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.
