Fig. 1: Pyroptosis level was significantly decreased in rhabdomyosarcoma.

A IHC was used to detect the expression of NLRP3, caspase-1, caspase-3, GSDMD and GSDME in RMS tissues and normal skeletal muscle tissues. Scale bar, 100 µm. B qRT-PCR assay was used to detect the mRNA expression of NLPR3, caspase-1, caspase-3, GSDMD and GSDME in RMS cells and skeletal muscle cells. C Western blot was used to detect the expression of NLPR3, caspase-1, caspase-3, GSDMD and GSDME in RMS cells and skeletal muscle cells.