Fig. 1: Ezrin is induced in monocytes and MΦs in response to LPS.

Quantitative PCR (qPCR) for ezrin in WT and Ezr-KO^m mouse bone marrow-derived macrophages (BMD-MΦs) (A) and primary bone marrow (BM) monocytes (B), untreated or treated with LPS for 6 h and 24 h. The relative mRNA expression of ezrin was normalized to STX5a and compared to WT untreated (Untr). Western blot (WB) and densitometric analysis for ezrin and moesin in mouse BMD-MΦs (C) and BM monocytes (D), untreated or treated with LPS for 6 h and 24 h. Protein fold increase was normalized to β-actin and relative to untreated cells. E Cartoon representation of the in vivo model of Pseudomonas aeruginosa—lipopolysaccharide (PA-LPS) nebulization. F qPCR of ezrin in murine WT and Ezr-KO^m monocyte-derived MΦs (moMs), interstitial MΦs (IMs) and alveolar MΦs (AMs), sorted from lung tissues of untreated or LPS treated mice. The relative ezrin mRNA expression was normalized to STX5a and the WT Untr., distinctly for each phenotype. Data are represented as mean ± SEM from three independent experiments with three or more mice per genotype. Statistical analysis was performed using one-way ANOVA or Tukey’s test for multiple comparisons between the genotype and treatment conditions. *p < 0.05, **p < 0.01 and ***p < 0.001. See also Supplementary Fig. S2.