Fig. 4: SQLE increased the sphere-forming potential of HGSOC cells. | Cell Death & Disease

Fig. 4: SQLE increased the sphere-forming potential of HGSOC cells.

From: Stabilization of SQLE mRNA by WTAP/FTO/IGF2BP3-dependent manner in HGSOC: implications for metabolism, stemness, and progression

Fig. 4

a Procedures for the stem cell screening and tumor sphere culture assay. b, c The SQLE level was higher in ALDH+CD133+ cells than in ALDH-CD133- cells. d Sphere-formation assay analysis of sphere-forming capacities of ALDH+CD133+ cells. The typical pictures of monolayer cells and tumorspheres were shown. The magnification of the pictures was 100×. e, f The qRT-PCR analysis of ALDH expression and CD133 expression in 2D cultured monolayer cell and tumor spheres. g, h The SQLE highly expressed in the tumor spheres than in monolayer cells. i ALDH+CD133+ cells with SQLE overexpression exhibited significantly higher sphere-forming capacity, and SQLE knockdown had the opposite effect. j, k SQLE enhances the expression of stemness markers ALDH and CD133. *p < 0.05, **p < 0.01, and ***p < 0.001.

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