Fig. 3: Deletion of LCN2 attenuated AP diet-induced CKD-VC in mice. | Cell Death & Disease

Fig. 3: Deletion of LCN2 attenuated AP diet-induced CKD-VC in mice.

From: Lipocalin-2 promotes CKD vascular calcification by aggravating VSMCs ferroptosis through NCOA4/FTH1-mediated ferritinophagy

Fig. 3

A Scheme of the experiment. After 2 weeks of acclimatisation, LCN2KO and WT mice were fed with a high adenine and phosphate diet (AP) or a normal diet (ND) for 14 weeks. B Survival curves of the experimental mice. Body weights (C), serum creatinine (D), BUN (E), serum phosphorus (F) and serum calcium levels (G) of the four groups (n = 5 per group). H Western-blot analysis and quantification (I) of LCN2 in the aorta (n = 5 per group). J Calcium concentrations of aortas (n = 5 per group). K Representative alizarin red stained images of the aorta. Scale bar:1 cm. L Representative Von Kossa staining of the aortic smooth muscle layer in the four groups and semi-quantification (M). Scale bar: 200 μm for 40×; 100 μm for 100×; 50 μm for 200×, 20 μm for 400×. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. CKD-VC chronic kidney disease-related vascular calcification, AP adenine and phosphate, KO knockout, WT wild type, BUN blood urea nitrogen, SD standard deviation.

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