Fig. 4: CPNE7 overexpression promotes the proliferation and migration of CRC cells.
From: CPNE7 promotes colorectal tumorigenesis by interacting with NONO to initiate ZFP42 transcription
A CPNE7 overexpression HT29 cell line was established and verified by Western blot. B Cell viability was measured by CCK-8 assay. C Colony formation assays of Vector and oeCPNE7 HCT116 cells. Representative images are shown on the left, and the statistical analysis is shown on the right. D Transwell assays of Vector and oeCPNE7 HCT116 cells. Representative images are shown on the left, and the statistical analysis is shown on the right. E Wound-healing assays of Vector and oeCPNE7 HCT116 cells. Representative images are shown on the left, and the statistical analysis is shown on the right. F Vector and oeCPNE7 HT29 cells were injected into BALB/c-nude mice (n = 5 mice per group). Volume changes of xenograft tumors in nude mice are shown. G Tumor images are shown on the left, and statistical analysis for tumor weights is shown on the right. H Expression of CPNE7 in xenograft tumors were detected by qRT-PCR. I IHC staining of CPNE7 and Ki-67 in xenograft tumors in (G). Scale bar: 100 μm. For (B–G), data are shown as mean ± SD and two-tailed unpaired Student’s t-test was used. *p < 0.05, **p < 0.01, ***p < 0.001. Data are representative of at least three independent experiments.
