Fig. 3: Inhibition of Sphk1 reduces degradation of tight junction (TJ) proteins after ICH in mice. | Cell Death & Disease

Fig. 3: Inhibition of Sphk1 reduces degradation of tight junction (TJ) proteins after ICH in mice.

From: Sphk1/S1P pathway promotes blood-brain barrier breakdown after intracerebral hemorrhage through inducing Nlrp3-mediated endothelial cell pyroptosis

Fig. 3

A-D Western blotting analysis of peri-hematomal TJ proteins levels in mice with ICH (n = 6 mice/group). E Immunofluorescence staining of Claudin-5 with endothelial cell maker CD31 at peri-hematomal area after ICH in mice. F Quantitative analysis of Claudin-5 fluorescence signal density normalized by CD31 area (n = 6 mice/group). G Immunofluorescence staining of Occludin with CD31 at peri-hematomal area after ICH in mice. H Quantitative analysis of Occludin fluorescence signal density normalized by CD31 area (n = 6 mice/group). I Immunofluorescence staining of ZO-1 with CD31 at peri-hematomal area after ICH in mice. J Quantitative analysis of ZO-1 fluorescence signal density normalized by CD31 area (n = 6 mice/group). K Transmission electron microscope of TJ proteins at peri-hematomal area after ICH in mice (Arrowheads indicate the opening TJ. L: lumen. EC: endothelial cell. BM: basement membrane. P: pericyte.). L Quantitative analysis of TJ opening percent. Data are expressed as means ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, one-way ANOVA and Tukey multiple comparisons test.

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