Fig. 4: Inhibition of Sphk1 decreases endothelial transcytosis after ICH in mice. | Cell Death & Disease

Fig. 4: Inhibition of Sphk1 decreases endothelial transcytosis after ICH in mice.

From: Sphk1/S1P pathway promotes blood-brain barrier breakdown after intracerebral hemorrhage through inducing Nlrp3-mediated endothelial cell pyroptosis

Fig. 4

A-C Western blotting analysis of peri-hematomal Mfsd2a/Caveolin-1 proteins levels in mice with ICH (n = 6 mice/group). D Immunofluorescence staining of Mfsd2a with endothelial cell maker CD31 at peri-hematomal area after ICH in mice. E Quantitative analysis of Mfsd2a fluorescence signal density normalized by CD31 area (n = 6 mice/group). F Immunofluorescence staining of Caveolin-1 with CD31 at peri-hematomal area after ICH in mice. G Quantitative analysis of Caveolin-1 fluorescence signal density normalized by CD31 area (n = 6 mice/group). H Transmission electron microscope of vesicles at peri-hematomal area after ICH in mice (Arrowheads indicated vesicles in endothelial cell. L: lumen. EC: endothelial cell. BM: basement membrane. P: pericyte.). I Quantitative analysis of vesicles density normalized by vessel perimeter length. Data are expressed as means ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, one-way ANOVA and Tukey multiple comparisons test.

Back to article page