Fig. 2: The effect of SIRT7 siRNA on the proliferation and cisplatin responsiveness of UMUC3 cells.

A Western blot analysis showing the expression levels of SIRT7 protein in SV-HUC-1 cells and various bladder cancer cell lines, GAPDH served as the internal control, and quantitative analyses are shown on the right. B CCK-8 assay was utilized to determine the effect of various concentrations of CDDP on the viability of different bladder cancer cell lines, and the IC50 value was determined. C Pearson correlation coefficient was calculated to assess the relationship between the IC50 values of CDDP and intracellular SIRT7 protein levels in bladder cancer cell lines. D Western Blot analysis was to determine the impact of NC siRNA or SIRT7 siRNA transfection on the intracellular SIRT7 protein levels in UMUC3 cells. The quantification graph of protein band is shown. E Western Blot analysis was conducted to evaluate the influence of SIRT7 siRNA and/or CDDP treatment on the intracellular SIRT7 protein content in UMUC3 cells. The graph on the right shows the expression levels of SIRT7 protein bands. F CCK-8 assay was used to determine the effect of SIRT7 siRNA and/or CDDP treatment on the activity of UMUC3 cells. G Plate colony assay results showing the impact of SIRT7 siRNA, CDDP, and their combination on the colony formation ability of UMUC3 cells. The number of colony cells is shown on the right. H Flow cytometry analysis of the apoptosis level of UMUC3 cells after treatment with NC siRNA or SIRT7 siRNA in combination with or without CDDP. The quantitative analysis graph is shown on the right. Quantitative data are presented as mean ± SD, n = 3. One-way ANOVA was used to assess group differences, with ns indicating p > 0.05 and ** indicating p < 0.01.