Fig. 1: TRIM59 is identified as a key regulator of the Notch signaling pathway in PC. | Cell Death & Disease

Fig. 1: TRIM59 is identified as a key regulator of the Notch signaling pathway in PC.

From: TRIM59/RBPJ positive feedback circuit confers gemcitabine resistance in pancreatic cancer by activating the Notch signaling pathway

Fig. 1

A The Notch signaling pathway was markedly altered in the PC dataset (GSE16515) of the GEO database verified by GSEA. B Relative expression level of RBPJ in the PC datasets of TCGA database. C RBPJ levels were assayed by IHC staining in PC and adjacent non-tumor tissues (n = 98). Scale bar, 100 μm. D The overall survival rate was assessed using Kaplan–Meier analysis with high/low RBPJ expression in PC patients. E Workflow of TRIM (s) screening using the 12× CSL-luciferase system. F Dual luciferase reporter assay was conducted to screen the TRIM candidates in PANC-1 cells. G, H The 12× CSL-luciferase activity was confirmed in MIA PaCa-2 (G) and SW1990 (H) cell lines using the dual luciferase reporter assay. I, J RT-qPCR (I) and western blotting (J) were performed to detect RBPJ, HES1, HEY1, MYC, SNAI1, and TRIM59 levels in PC cells. *P < 0.05, **P < 0.01, ***P < 0.001, ns no significance.

Back to article page