Fig. 2: Inhibition of KLF5 promotes ferroptosis in pancreatic cancer cells. | Cell Death & Disease

Fig. 2: Inhibition of KLF5 promotes ferroptosis in pancreatic cancer cells.

From: Inhibition of KLF5 promotes ferroptosis via the ZEB1/HMOX1 axis to enhance sensitivity to oxaliplatin in cancer cells

Fig. 2: Inhibition of KLF5 promotes ferroptosis in pancreatic cancer cells.The alternative text for this image may have been generated using AI.

A The lollipop diagram depicted the correlation between KLF5 and ten key genes related to ferroptosis according to mRNA expression using Spearman analysis. The patients with pancreatic cancer come from three datasets: TCGA-PAAD, GEO (GSE57495), and GEO (GSE71729). B KEGG analysis of KLF5-targeted genes. C qRT-PCR evaluated knockdown efficiency of shKLF5. WB evaluated the knockdown efficiency of shKLF5. D Heatmap depicted inhibition of KLF5 increased the sensitivity to ferroptosis inducers RSL3 and Erastin. E Short hairpin RNA was used to silence KLF5 in CFPAC-1 and Capan-1 cells. The cells were treated with Fer-1 (2 μmol/L) for 16 h. Cell viability was detected by calcein-AM. F BODIPY 581/591 C11 was used to detect lipid peroxidation in CFPAC-1 and Capan-1 cells. G Fluorescence was calculated. H Confocal imaging revealed the effect of inhibition of KLF5 on lipid peroxidation. I MDA level was analyzed in KLF5-silenced CFPAC-1 and Capan-1 cells in the presence or absence of 2 μmol/L Fer-1. J Evaluation of shKLF5-treated tumors with or without Fer-1 (30 mg/kg) treatment. K MDA level in tumors. L Evaluation of ML264-inhibited tumors with or without Fer-1 (30 mg/kg) treatment. M MDA levels in tumors.

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