Fig. 5: HSP70 facilitates the physical interaction between CREB1 and CREBBP.
From: Pifithrin-μ sensitizes mTOR-activated liver cancer to sorafenib treatment
A Immunoblotting of SNU886 or SNU398 cells transfected with control or mTOR shRNA. B, C SNU886 cells were treated with SGC-CBP30 (5 μM) for 24 h. mRNA levels of CREB1 target genes, n = 3 (B). Immunoblotting of cells (C). D Confocal images illustrating the co-localization of CREB1 and CREBBP in SNU886 and SNU398 cells. Scale bar = 10 μm. E, F Co-IP demonstrating the interaction of CREB1 and CREBBP in SNU886 (E) and SNU398 (F) cells. G, H Confocal images showing the interaction of HSP70 with CREB1 (G) or CREBBP (H) in SNU886 and SNU398 cells. Scale bar = 10 μm. I, J Co-IP experiments showing the interaction of HSP70, CREB1, and CREBBP in SNU886 (I) and SNU398 (J) cells. K Co-IP analysis for CREB1 interaction with CREBBP after silencing HSP70 in SNU886 cells. Data are displayed as mean ± SD (error bars). n.s., not statistically significant, **p < 0.01, ***p < 0.001.
