Table 1 Primary antibodies.
Name of antibody | Host | Fluorochrome | Manufacturer, catalog number | Application | Working dilutions or concentrations |
|---|---|---|---|---|---|
CD45 | Mouse | APC | Biolegend, 103112 | Flow Cytometry | 1:200 |
CD11b | Mouse | FITC | Biolegend, 101206 | Flow Cytometry | 1:500 |
Ly6G | Mouse | PE | Biolegend, 127608 | Flow Cytometry | 1:200 |
Ly6G | Mouse | Percp-cy5.5 | Biolegend, 127616 | Flow Cytometry | 1:200 |
Ly6C | Mouse | Percp-cy5.5 | Biolegend, 128012 | Flow Cytometry | 1:200 |
Ly6C | Mouse | BV605 | Biolegend, 128035 | Flow Cytometry | 1:200 |
CX3CR1 | Mouse | PE-cy7 | Biolegend, 149016 | Flow Cytometry | 1:200 |
TREM-1 | Mouse | PE | Invitrogen, MA5-28221 | Flow Cytometry | 1:400 |
Iba1 | Mouse | - | Wako, 019-19741 | IF | 1:500 |
Hexb | Rabbit | - | Proteintech, 16229-1-AP | IF | 1:200 |
TH | Mouse | - | Abcam, ab217161 | IF | 1:400 |
WB | 1:2000 | ||||
TNF-α | Rabbit | - | Proteintech, 1590-1-AP | WB | 1:1000 |
TREM-1 | Mouse | - | Novusbio | WB | 1:500 |
IL-1β | Rabbit | - | Proteintech, 26048-1-AP | WB | 1:1000 |
IL-6 | Rabbit | - | Proteintech, 29444-1-AP | WB | 1:1000 |
β-actin | Rabbit | - | Proteintech, 66009-1-Ig | WB | 1:2000 |
Tubulin | Rabbit | - | Affinity, DF7967 | WB | 1:3000 |
