Fig. 3: Mitophagy and mitochondrial biogenesis in preconditioned neurons exposed to OGD and OGD/REOXY. | Cell Death & Disease

Fig. 3: Mitophagy and mitochondrial biogenesis in preconditioned neurons exposed to OGD and OGD/REOXY.

From: The E3-ligase Siah2 activates mitochondrial quality control in neurons to maintain energy metabolism during ischemic brain tolerance

Fig. 3: Mitophagy and mitochondrial biogenesis in preconditioned neurons exposed to OGD and OGD/REOXY.The alternative text for this image may have been generated using AI.

A Siah2, B LC3-II, C PGC-1α proteins expression measured by western blot analysis in cortical neurons exposed to IPC and IPC followed by OGD/REOXY. Each bar represents the mean ± S.E.M. of the percentage of different experimental values obtained in three independent experimental sessions. *P < 0.05 vs CTL; #P < 0.05 vs its relative ischemic stimulus. D Colocalization between LC3-GFP (green) and Mito-RFP (red) in cortical neurons exposed to IPC and IPC followed by OGD/REOXY. LC3-GFP/Mito-RFP colocalization was 100% in CTL condition, 154% ± 1 after 30 min IPC, 112% ± 2 after IPC + 48 h RX, 173% ± 1 after 3 h of OGD, 124% ± 3 after OGD/REOXY, 161% ± 3 after 30 min IPC + OGD, 107% ± 3 after 30 min IPC + OGD RX. Bars 10 μM.

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