Fig. 4: PLIN1 suppresses glioma progression through regulating lipid metabolism.

A KEGG analysis in the above 33 glioma samples and 15 brain normal samples was shown. B GSEA analysis presented the relationship between glycerophospholipid metabolism and glioma tumorigenesis. C GSEA analysis presented the relationship between fat cell differentiation and glioma tumorigenesis. D Oil red O staining conducted to detect intracellular lipid contents after PLIN1 overexpression in glioma cell lines (Left). Intracellular lipid droplet contents were quantified by ImageJ software (Right). E The intracellular TG levels were detected after PLIN1 overexpression. F The intracellular FFA levels were detected after PLIN1 overexpression. G Lipid metabolism-related genes levels were determined by western blotting. The western blotting bands were quantified with ImageJ software (Right). *P < 0.05, **P < 0.01. The experiment was repeated three times independently.