Fig. 2: Ferroptosis inhibitor Ferrostatin-1 attenuates, whereas Erastin enhances ferroptosis induced by knockdown of ERα. | Cell Death & Disease

Fig. 2: Ferroptosis inhibitor Ferrostatin-1 attenuates, whereas Erastin enhances ferroptosis induced by knockdown of ERα.

From: Targeting estrogen-regulated system xc− promotes ferroptosis and endocrine sensitivity of ER+ breast cancer

Fig. 2: Ferroptosis inhibitor Ferrostatin-1 attenuates, whereas Erastin enhances ferroptosis induced by knockdown of ERα.

A, B Ferrostatin-1 partially inhibited cell ferroptosis caused by ERα knockdown. ERα-knocked down MCF-7 (A) and ZR-75-1 (B) cells were treated with vehicle or Ferrostatin-1 (5 μM) alone for 24 h. Lipid ROS were detected by flow cytometry. C, D Ferrostatin-1 treatment rescued the decrease in cell growth induced by ERα knockdown in MCF-7 (C) and ZR-75-1 (D) cells. Cells were treated with Ferrostatin-1 (5 μM) for 72 h before being analyzed using the CCK8 reagent. E, F The ferroptosis inducer Erastin enhanced cell ferroptosis induced by ERα knockdown. ERα-knocked down MCF-7 (E) and ZR-75-1 (F) cells were treated with vehicle and Erastin (10 μM) alone for 24 h. Lipid ROS were detected by flow cytometry. G, H Erastin treatment enhanced the decrease in cell growth induced by ERα knockdown. MCF-7 (G) and ZR-75-1 (H) cells were treated with Erastin (10 μM) for 72 h before being analyzed using the CCK8 reagent. Data are shown as Mean ± SEM (n = 3). *P < 0.05, ***P < 0.001, ###P < 0.001.

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