Fig. 2: FBXO33 regulates EMT progression of GBC in vitro and in vivo. | Cell Death & Disease

Fig. 2: FBXO33 regulates EMT progression of GBC in vitro and in vivo.

From: Lactylation-driven transcriptional activation of FBXO33 promotes gallbladder cancer metastasis by regulating p53 polyubiquitination

Fig. 2: FBXO33 regulates EMT progression of GBC in vitro and in vivo.The alternative text for this image may have been generated using AI.

A Western blot was used to detect the knockdown effect of sh-FBXO33. B Western blot was utilized to examine the overexpression effect of Flag-FBXO33. C, D Transwell chamber assay and scratch assay were performed to evaluate the impact of sh-FBXO33 on the migration ability of GBC cells. E, F Scratch assay and Transwell chamber assay were conducted to assess the effect of Flag-FBXO33 on the migration ability of GBC cells. G Western blot detected changes in E-Cadherin and Vimentin protein levels in GBC cells after FBXO33 knockdown. H Western blot detected changes in E-Cadherin and Vimentin protein levels in GBC cells after FBXO33 overexpression. I Impact of sh-FBXO33 on subcutaneous tumor size in GBC. Impact of sh-FBXO33 on subcutaneous tumor (J) weight and (K) volume in GBC. L IHC analysis of the effect of sh-FBXO33 on the protein expression of E-Cadherin and Vimentin in GBC subcutaneous tumors. Error bars represent the mean (n = 3) ±SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

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