Fig. 7: Transcription factor YY1 promotes FBXO33 expression in GBC. | Cell Death & Disease

Fig. 7: Transcription factor YY1 promotes FBXO33 expression in GBC.

From: Lactylation-driven transcriptional activation of FBXO33 promotes gallbladder cancer metastasis by regulating p53 polyubiquitination

Fig. 7: Transcription factor YY1 promotes FBXO33 expression in GBC.The alternative text for this image may have been generated using AI.

A Bioinformatics predicted YY1 as a potential transcription factor of FBXO33. B Western blot detected the knockdown effect of sh-YY1. C Western blot examined the overexpression effect of OE-YY1. RT-qPCR detected the impact of sh-YY1 (D) and OE-YY1 (E) on FBXO33 mRNA levels in GBC cells. Western blot assessed the effect of sh-YY1 (F) and OE-YY1 (G) on FBXO33 protein levels in GBC cells. H Schematic diagram of potential binding sequences of YY1 to the FBXO33 promoter region (WT-PRO and MUT-PRO plasmids). WT-PRO and MUT-PRO plasmids were transfected into corresponding GBC cells, and after 48 h, a dual-luciferase reporter assay was conducted to assess the effect of sh-YY1 (I) and OE-YY1 (J) on fluorescence values in WT-PRO group and MUT-PRO group. ChIP-qPCR detected the enrichment of YY1 in the FBXO33 promoter region in sh-YY1 (K) and OE-YY1 (L) groups. Error bars represent the mean (n = 3) ±SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

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