Fig. 5: CXCL1 promotes fatty acid synthesis in hepatocytes by binding to CXCR2. | Cell Death & Disease

Fig. 5: CXCL1 promotes fatty acid synthesis in hepatocytes by binding to CXCR2.

From: Smad4 deficiency in hepatocytes attenuates NAFLD progression via inhibition of lipogenesis and macrophage polarization

Fig. 5: CXCL1 promotes fatty acid synthesis in hepatocytes by binding to CXCR2.The alternative text for this image may have been generated using AI.

Groups of Smad4fl/fl and AlbSmad4−/− mice were fed an HFD for 4 months to establish the NAFLD model (n = 5 per group). A The mRNA levels of ACC1, FASN, SCD1, FABP1, FATP1, ACOX1, and CPT1a in NAFLD liver tissues were measured using RT-qPCR analysis *P < 0.05 and **P < 0.01. B–G Primary hepatocytes and AML12 cells were treated with BSA or PA (500 μM) for 24 h. B The mRNA levels of ACC1, FASN, and PPARγ in primary hepatocytes were determined using RT-qPCR analysis *P < 0.05 and ***P < 0.001. C Oil Red O staining of primary hepatocytes. Scale bar, 100 μm. D The mRNA levels of ACC1, FASN, and SCD1 were determined using RT-qPCR analysis in AML12 cells with si-NC or si-Smad4 transfection *P < 0.05 and **P < 0.01. E Oil Red O staining of AML12 cells transfected with si-NC or si-Smad4. Scale bar, 10 μm. F The mRNA levels of ACC1, FASN, and SCD1 were determined using RT-qPCR analysis in AML12 cells transfected with sh-GFP or sh-Smad4 *P < 0.05 and **P < 0.01. G Oil Red O staining of AML12 cells transfected with sh-GFP or sh-Smad4, respectively. Scale bar, 10 μm. H The protein levels of CXCR2 in AML12 cells treated with 50 ng/mL CXCL1 were detected by Western blot. I AML12 cells were cultured with 500 μM PA, 50 ng/mL CXCL1 recombinant protein, and 500 nM SB225002 (inhibitor of CXCL1 receptor CXCR2) for 24 h. The mRNA levels of ACC1, FASN, and SCD1 were determined using RT-qPCR analysis in AML12 cells *P < 0.05, **P < 0.01 and ***P < 0.001.

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