Fig. 6: CXCL1 promotes macrophage M1 polarization. | Cell Death & Disease

Fig. 6: CXCL1 promotes macrophage M1 polarization.

From: Smad4 deficiency in hepatocytes attenuates NAFLD progression via inhibition of lipogenesis and macrophage polarization

Fig. 6: CXCL1 promotes macrophage M1 polarization.The alternative text for this image may have been generated using AI.

Smad4fl/fl and AlbSmad4−/− mice were fed an HFD for 4 months to establish the NAFLD model (n = 5 per group). Data are representative of at least three independent experiments. A, B Representative staining and statistical analysis of CD86 and CD206 expression in liver tissues Scale bar, 50 μm. C, D The mRNA levels of IL-6, MCP1, TNF-α, Arg1, IL-10, and YM1 in liver tissues were determined using RT-qPCR analysis *P < 0.05 and **P < 0.01. E The protein levels of CXCR2 in Raw264.7 cells treated with 50 ng/mL CXCL1 were detected by Western blot. F, G Raw264.7 cells were cultured with 100 ng/mL LPS, 10 ng/mL IFN-γ, 50 ng/mL CXCL1 recombinant protein, and 500 nM SB225002 for 24 h. F Immunofluorescence staining of CD86 in RAW264.7 cells Scale bar, 50 μm. G The mRNA levels of iNOS, MCP1, and TNF-α in RAW264.7 cells were determined using RT-qPCR analysis *P < 0.05, **P < 0.01 and ***P < 0.001. H Raw264.7 cells were cultured with 20 ng/mL IL-4, 20 ng/mL IL-13, 50 ng/mL CXCL1 recombinant protein, and 500 nM SB225002 for 48 h. The mRNA levels of Arg1, IL-10, and YM1 in RAW264.7 cells were determined using RT-qPCR analysis ***P < 0.001.

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