Fig. 2: Both combined treatment of NCT-503 and perhexiline/ALPI3MT55 lead to apoptotic cell death.
From: PHGDH inhibition and FOXO3 modulation drives PUMA-dependent apoptosis in osteosarcoma
A Expression of cleaved caspase 3 under the combined treatments in OS cells. B Perhexiline treatment induced capase3/7-dependent apoptotic cell death with PHGDH inhibition, and pan caspase inhibitor Z-VAD-FMK repressed the cell death induction caused by the combined treatment. C ALPI3MT55 also induced apoptosis with PHGDH inhibition in OS cells. All experiments are n = 3 at least. Bars represent means of values; error bars represent SEM. *P < 0.05, **P < 0.01, ***p < 0.005, ****p < 0.001.
