Fig. 3: Combined inhibition of PHGDH and mTORC1 with non-rapalog mTORC1 inhibitors elevate the transcription of the pro-apoptotic gene PUMA.
From: PHGDH inhibition and FOXO3 modulation drives PUMA-dependent apoptosis in osteosarcoma
A The mRNA expression of PUMA in OS cells under the combined treatments. B Protein expression of PUMA under the combined treatments. C Rapamycin treatment did not promote PUMA transcription of OS cells with NCT-503. D Protein expression of PUMA in PUMA KO OS cells. E Expression of cleaved caspase 3 protein in PUMA KO OS cells under the combined treatment. Percentage of cell death of PUMA KO OS cells under the combined treatment of NCT-503 and perhexiline (F) and the combined treatment of NCT-503 and ALPI3MT55 (G). All experiments are n = 3 at least. Bars represent means of values; error bars represent SEM. *P < 0.05, **P < 0.01, ***p < 0.005, ****p < 0.001.
