Fig. 5: NCT-503, perhexiline, and ALPI3MT55 promote the nuclear localization of FOXO1/3, with FOXO3 being dominant in inducing cell death in OS cells under the combined treatments.
From: PHGDH inhibition and FOXO3 modulation drives PUMA-dependent apoptosis in osteosarcoma
A The phosphorylation of FOXO of OS cells under the combined treatments. Cytosolic/nuclear localization of FOXO1 protein of NOS1 cells under the combined treatment of NCT-503 and perhexiline (B) or ALPI3MT55 (C). Cytosolic/nuclear localization of FOXO3 protein of NOS1 cells under the combined treatment of NCT-503 and perhexiline (D) or ALPI3MT55 (E). F FOXO1 and FOXO3 knockouts in OS cells. G mRNA expression of PUMA in FOXO1 KO, FOXO3 KO, or FOXO1/3 double KO cells under the combined treatments. All experiments are n = 3 at least. Bars represent means of values; error bars represent SEM. *P < 0.05, **P < 0.01, ***p < 0.005, ****p < 0.001.
