Fig. 1: Highly myopic lenses are more sensitive to ferroptosis. | Cell Death & Disease

Fig. 1: Highly myopic lenses are more sensitive to ferroptosis.

From: Enhanced ferroptosis sensitivity promotes the formation of highly myopic cataract via the DDR2-Hippo pathway

Fig. 1

a–d Evaluation of intrinsic Fe2+ and lipid peroxidation levels in lens epithelia from highly myopic cataract (HMC) and age-related cataract (ARC) patients (n = 3). For the FerroOrange, mean gray values were normalized to the average of the ARC group. For the C11/BODIPY, the ratio of gray values between the FITC and TRITC channels was calculated to represent the degree of lipid peroxidation. Scale bars: 100 μm. e Mitochondria observed using transmission electron microscopy in epithelia from HMC and ARC patients. Cells were treated with 1 μM RSL3 or DMSO for 4 h before being fixed. Red arrows show the mitochondria. Scale bars: 500 nm and 200 nm. f–i Assessment of Fe2+ (f, g) and lipid peroxidation (h–i) levels in lens epithelia of highly myopic and the contralateral eyes of mice (n = 3). Scale bars: 100 μm. j, k Representative photos and quantification of lens opacity from highly myopic and the contralateral eyes of mice before and after 10 μM RSL3 treatment. The opacity area (%) is defined as the ratio of the projection area of nuclear opacification to that of the whole lens. Data are presented as mean ± SD. Significance level was determined using unpaired t-tests (b, d) and paired t-tests (g, i, k). *P < 0.05, **P < 0.01, ***P < 0.001.

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