Fig. 3: DDR2 enhances the ferroptosis sensitivity of lens epithelial cells. | Cell Death & Disease

Fig. 3: DDR2 enhances the ferroptosis sensitivity of lens epithelial cells.

From: Enhanced ferroptosis sensitivity promotes the formation of highly myopic cataract via the DDR2-Hippo pathway

Fig. 3

a–c Quantitative PCR (a) and Western blotting (b, c) analyses display the mRNA and protein levels of DDR2 after transduction using the DDR2 overexpressing lentivirus (qPCR, n = 5; Western blotting, n = 3). d Cell viability of DDR2-OE cells assessed by CCK-8 after 24 h treatment with varying concentrations of Erastin and RSL3 (n = 5). e–j Measurements of reactive oxygen species (e), lipid peroxidation (f, g), Fe2+ (h, i), and malondialdehyde (MDA) (j) levels using DCFH-DA probe, C11-BODIPY staining (n = 5), FerroOrange staining (n = 5) and an MDA assay kit (n = 5), respectively. Control and DDR2-OE cells were pretreated with 1 μM Erastin or RSL3 for 24 h. The quantification methods of FerroOrange and C11-BODIPY staining are as same as the description in Fig. 1. Scale bars: 100 μm. Band densities for Western blotting were normalized to the GAPDH for statistical analysis. Data are presented as mean ± SD. Multiple unpaired t-tests with False Discovery Rate post comparisons (d) and unpaired t-tests (a, c, g, i, j) were used. *P < 0.05, **P < 0.01, ****P < 0.0001.

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