Fig. 2: NOL9 modulates HCC cell proliferation and apoptosis.

A CCK-8 assay showing the proliferation of HCC cells. B EdU assay showing the proliferation of HCC cells. C Clonogenic assay showing the colony formation ability of HCC cells. D Flow cytometry assay showing the percentage of apoptotic HCC cells. E Huh7 cells with stable NOL9 knockdown were subcutaneously injected into the right flanks of nude mice (n = 3 per group). Tumor volumes and tumor weights on day 30 post-transplantation were measured. F Western blotting analysis of NOL9 expression. *p < 0.05, **p < 0.01, ***p < 0.001.