Fig. 7: Piezo1 is necessary for neutrophil NET formation and macrophage differentiation during virus infection.

Wild-type (WT) cells were infected with the PR8 virus for 48 h and treated with or without GsMTx4 (2.0 mg/kg, MCE). A Haematoxylin and eosin (H&E) staining of infected mouse lungs. B Expression of ROS in neutrophils isolated from the BALF and lungs by flow cytometry. Dot plots present representative data (left) and a summary of the data (right). C Western blot analysis of PAD4 in neutrophils isolated from the BALF and lungs. NETs from neutrophils isolated from the BALF (D) and lungs (E) were detected via confocal fluorescence microscopy. Typical NET images are displayed. Scale bars, 50 µm; original magnification, 630X. F Intracellular staining of TNFα, IL-10 and NOS2 and the expression of CD206 in CD11b⁺F4/80⁺ macrophages isolated from the BALF of virus-infected mice were measured via flow cytometry. Fluorescence Minus One control, FMO. Dot plots present representative data from flow cytometry analysis (upper), and the statistical results are shown (lower). The graph shows data from three independent experiments with four mice per group. *P < 0.05 and ***P < 0.001, compared with the indicated groups.