Fig. 5: BRD1-related SREBF1 expression regulation is dependent on epigenetic regulation of H3K9 and H3K14 loci. | Cell Death & Disease

Fig. 5: BRD1-related SREBF1 expression regulation is dependent on epigenetic regulation of H3K9 and H3K14 loci.

From: BRD1 deficiency affects SREBF1-related lipid metabolism through regulating H3K9ac/H3K9me3 transition to inhibit HCC progression

Fig. 5

A Immunoblotting was performed to assess the total protein levels of H3K14ac and H3K9ac in Huh7 and Hep3B cells. B The integrative genomics viewer (IGV) was used to visualize the genomic enrichment of H3K14ac and BRD1 across all cells, based on data retrieved from the ChIP-Atlas database. Shaded areas Shaded areas highlight the enrichment of H3K9ac and H3K14ac specifically at the SREBF1 promoter region. C, D The enrichment of H3K14ac, H3K9ac, H3K9me3, and SETDB1 in the SREBF1 promoter region was detected by ChIP-qPCR. The figures on the right depict the band size of SREBF1, detected by nucleic acid electrophoresis using shNC and shBRD1 ChIP-DNA as templates. The product length observed is 98 base pairs (bp). Data represent the mean ± SD of three independent experiments. ns p > 0.05; *p < 0.05; **p < 0.01.

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