Fig. 6: ShRNA-Blimp-1 altered metabolome in M2 macrophages. | Cell Death & Disease

Fig. 6: ShRNA-Blimp-1 altered metabolome in M2 macrophages.

From: Blimp-1 orchestrates macrophage polarization and metabolic homeostasis via purine biosynthesis in sepsis

Fig. 6

RAW264.7 cells were induced by IL-4 and IL-13 to polarize towards M2 macrophages, followed by infection with lentivirus carrying shRNA-NC or shRNA-Blimp-1. A Classification of the 121 metabolites identified in non-targeted metabolome analysis. B OPLS-DA Model Discrimination for non-targeted metabolome. C Heatmap of all metabolites for quantitative identification in the non-targeted metabolome. The color scale indicates the level of metabolite accumulation, while the class indicates the metabolites. D VIP scores comparing shRNA-Blimp-1 and shRNA-NC groups in non-targeted metabolome analysis. The green dots represented metabolites with VIP < 1.0 & |Corr.Coeffs. | < 0.9; the blue dots represented metabolites with VIP ≥ 1.0, and 0.9 ≤ |Corr.Coeffs.| < 1.0; the red dots represented metabolites with VIP ≥ 1.0, and |Corr.Coeffs.| ≥ 1.0. E OPLS-DA Model Discrimination for nucleotide-targeted metabolome. F OPLS-DA Model Discrimination for amino acid-targeted metabolome. G SMPDB metabolic pathway enrichment analysis of the differential metabolites in the nucleotide-targeted metabolome (P < 0.05). H KEGG metabolic pathway enrichment analysis of the differential metabolites in the amino acid-targeted metabolome (P < 0.05).

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