Fig. 6: IL-15 complex treatment restores NK maturation.
From: Regulation of NK cell development, maturation, and antitumor responses by the nuclear receptor NR2F6
A Schematic overview of the experimental setup of IL-15/IL-15Rα-Fc treatment. Wild-type (Nr2f6+/+) or Nr2f6-deficient (Nr2f6−/−) mice were injected 3 x with IL-15/IL-15Rα-complex or PBS i.v. Mice were analyzed nine days after the first injection. B Quantification of total cell counts and frequencies of splenic NK cells (CD45+CD3-CD19-NK1.1+NKp46+) in wild-type (Nr2f6+/+) or Nr2f6-deficient (Nr2f6−/−) mice after IL-15/IL-15Rα-Fc or PBS injection. C Representative dot-plots of total splenic NK cells (CD45+CD3-CD19-NK1.1+NKp46+), maturation (CD27-CD11b-, CD27+CD11b-, CD27+CD11b+, CD27-CD11b+), and terminally matured (CD27-CD11b+ KLRG1+) NK cell populations in wild-type (Nr2f6+/+) or Nr2f6-deficient (Nr2f6−/−) mice after IL-15/IL-15Rα-Fc injection. D Quantification of NKp46 expression (MFI) in splenic NK cells (CD45+CD3-CD19-NK1.1+NKp46+) of wild-type (Nr2f6+/+) or Nr2f6-deficient (Nr2f6−/−) mice after IL-15/IL-15Rα-Fc or PBS injection. E Quantification of frequencies of CD27+CD11b-, CD27+CD11b+ and mature CD27-CD11b+ splenic NK cell (CD45+CD3-CD19-NK1.1+NKp46+) in wild-type (Nr2f6+/+) or Nr2f6-deficient (Nr2f6−/−) mice after IL-15/IL-15Rα-Fc or PBS injection. F Quantification of the frequency of terminally matured (CD27-CD11b+KLRG1+) NK cells (CD45+CD3-CD19-NK1.1+NKp46+) in wild-type (Nr2f6+/+) or Nr2f6-deficient (Nr2f6−/−) mice after IL-15/IL-15Rα-Fc or PBS injection. B, E–F Representative data are shown as pooled experiments of five independent experiments n = 8 (PBS treated wild-type (Nr2f6+/+) or Nr2f6-deficient (Nr2f6−/−) mice) and n = 15 (IL15C treated wild-type (Nr2f6+/+) mice) or n = 16 (IL-15C treated Nr2f6-deficient (Nr2f6−/−) mice). D The representative data shown are from one independent experiment out of five replicative experiments, with n = 2-4 per group and experiment. Each dot represents the data of an individual mouse. Results are shown as mean ± SD. The normality of data was evaluated by the Shapiro–Wilk test. An asterisk indicates statistically significant differences between genotypes calculated using Student’s t-test or Mann-Whitney U test. A p value < 0.05 was considered statistically significant. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001;.
