Fig. 1: Dysregulated low levels of MAGI3 correlate with poor prognosis in ccRCC.

A, B Immunohistochemical (IHC) analysis of MAGI3 protein in ccRCC specimens. Representative images of MAGI3 staining in adjacent tissues or tumors (A). Scale bars: 200 μm. Magnified images of dashed areas are displayed in the right panels. Scale bars: 50 μm. Dot plot showing the corresponding quantification of the MAGI3 H-score (B). Data presented as mean ± SEM. Statistical significance was calculated by the Mann–Whitney test. ***P < 0.001. C Dot plot comparing the mRNA expression level of MAGI3 in TCGA ccRCC specimens with paired normal tissues. Statistical significance is determined using the paired t-test (****P < 0.0001). D–F TCGA ccRCC patients were stratified into two groups based on ccRCC specimens MAGI3 expression cutoff at 261.5 (reads per kilobase of transcript, per million mapped reads, RPKM): high and middle-to-low (M/L). Kaplan–Meier survival plots for overall survival (OS) (D), progression-free survival (PFS) (E) and disease-specific survival (DSS) (F) of the two groups (log-rank test). G Dot plot showing the mRNA expression level of MAGI3 in tumor tissues and adjacent tissues of TCGA ccRCC patients with stage I. Statistical significance was calculated using the unpaired t-tests (****P < 0.0001). Kaplan–Meier survival plots for OS (H), PFS (I) and DSS (J) according to MAGI3 mRNA level in tumor tissue of TCGA ccRCC patients with stage I (log-rank test). K, L Prognostic analysis of OS according to MAGI3 histochemistry score (H-score) in tumor tissue of ccRCC patients with stage I from Chinese cohort. Representative images with different MAGI3 H-scores (K). Scale bars: 200 μm. The magnified images are displayed in the right panels. Scale bars: 50 μm. Kaplan–Meier survival plots for OS of patients with MAGI3 at high levels (H-score ≥ 8) and MAGI3 at middle-to-low levels (M/L, H-score ≤ 6) (log-rank test) (L).