Fig. 3: MAGI3 inhibits proliferation of ccRCC cells.

A MAGI3 overexpression was confirmed via Western blot analysis in 786-O and 769-P cells following transfection with lentivirus carrying MAGI3 coding sequence or control vectors. B Increased MAGI3 expression reduced cell viability in 786-O and 769-P cells, as assessed over a 5-day period using a CCK8 assay. C, D Upregulated MAGI3 expression decreased colony formation ability, evident in crystal violet staining and photographs of ccRCC cell colonies formed over a 10-day culture period (C). Quantitative analysis shows a reduced number of colonies (D). E MAGI3 knockdown was confirmed in 786-O and 769-P cells using lentivirus-mediated delivery of shRNA, as evidenced by Western blot analysis. F Decreased MAGI3 expression enhanced cell viability, observed over a 5-day period through CCK8 assays in 786-O and 769-P cells. G, H Reduced MAGI3 expression increased colony formation in ccRCC cells, as observed through crystal violet staining and colony photography (G). Quantitative analysis indicated an increased number of colonies (H). I, J The inhibitory effect of MAGI3 on cell proliferation was abolished by blocking the MAS receptor with A779, as demonstrated in CCK8 (I) and cell colony formation (J) assays in MAGI3-overexpressing 786-O cells and control cells treated with A779. Statistical significance is indicated by ***(P < 0.001); NS non-significant.