Fig. 2: LONP1 promotes proliferation, migration, and invasion in PCa cells.
From: Lon protease 1-mediated metabolic reprogramming promotes the progression of prostate cancer
A Immunoblotting of LONP1 in PC3 cells expressing shRNA against LONP1 or vector control. B Immunoblotting of LONP1 in DU145 cells stably transfected with control plasmid (pCDH-CMV-MCS-EF1-copGFP) or LONP1 plasmid (pCDH-CMV-MCS-EF1-copGFP-LONP1). C, D Cell viability of PC3 with LONP1 knockdown (C) and DU145 with LONP1 overexpression (D) were determined by CCK-8 assay. E, F Colony-formation assay of PC3 cells with LONP1 knockdown (E) and DU145 cells with LONP1 overexpression (F). Left: representative images of colony formation; right: quantitative analysis of colony numbers per well. G, H Migration and invasion assays of LONP1-knockdown PC3 cells (G) and GFP-LONP1 expressing DU145 cells (H). Upper: representative images of migratory and invasive cells; lower: quantitative analysis of cell numbers. Scale bar: 200 μm. Data are represented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.
