Fig. 5: OGT physically interacted with BAP1 resulting in nuclear degradation of BAP1. | Cell Death & Disease

Fig. 5: OGT physically interacted with BAP1 resulting in nuclear degradation of BAP1.

From: Nuclear localization of BRCA1-associated protein 1 is important in suppressing hepatocellular carcinoma metastasis via CTCF and NRF1/OGT axis

Fig. 5: OGT physically interacted with BAP1 resulting in nuclear degradation of BAP1.

A GST pull-down assay with silver staining using BAP1-GST fusion protein. Blue arrowheads indicate GST protein, and red arrowheads indicate BAP1-GST fusion proteins. Orange arrowheads indicate the differential bands in the group of BAP1-GST fusion protein incubated with PLC/PRF/5 nuclear protein, as compared with BAP1-GST fusion protein only group (left panel). The list of the human proteins fished out by BAP1-GST fusion protein upon incubation with PLC/PRF/5 nuclear proteins, as compared with GST protein alone (right panel). B Co-immunoprecipitation (co-IP) assay using anti-BAP1 antibody and nuclear protein compartment of PLC/PRF/5. C Overexpression of OGT reduced the BAP1 protein levels in PLC/PRF/5 cells. D Knockdown of OGT enhanced the BAP1 protein levels. E Immunofluorescence staining of BAP1 upon knockdown OGT in PLC/PRF/5 cells. Arrows indicated BAP1 localized at nucleus upon knockdown OGT. Scale bar, 50 μm. F O-linked-N-acetylglucosamine (O-GlcNAc) modification status of nuclear BAP1 protein in PLC/PRF/5 cells using anti-O-GlcNAc antibody detection. Arrows indicate the corresponding BAP1 protein.

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