Fig. 1: The expression of HNF4A in lung tissues of septic mice is down-regulated. | Cell Death & Disease

Fig. 1: The expression of HNF4A in lung tissues of septic mice is down-regulated.

From: HNF4A mitigates sepsis-associated lung injury by upregulating NCOA2/GR/STAB1 axis and promoting macrophage polarization towards M2 phenotype

Fig. 1

A Scheme to establish the mouse model of sepsis by cecal ligation and puncture (CLP). Schematics are obtained from SciDraw. Mouse: https://doi.org/10.5281/zenodo.3926105; Petrified Dish:10.5281/zenodo.6808892; Falcon Tube: 10.5281/zenodo.4421153; Syringe: 10.5281/zenodo.4152947. B The survival probability of mice is measured. C Comparison of wet/dry weight ratio of lung tissues of mice in sham surgery mice and CLP mice. D Hematoxylin and eosin (H&E) staining (×200) of lung tissues and lung damage scores in sham surgery mice and CLP mice. E The expression of HNF4A is assayed by real-time PCR and western blot (WB) in lung tissues. F Immunofluorescence (IF) staining (×400) of F4/80 and HNF4A shows the expression of HNF4A in alveolar macrophages. G The expression of HNF4A is assayed by real-time PCR and WB in alveolar macrophages. The data are presented as mean ± SD. **P < 0.01, ****P < 0.0001.

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